Developing best practices for measurement of oceanic 15N2 fixation rates and relationships to communities - PIs: Angelicque White (Oregon State Univ.), Pia Moisander (UMass Dartmouth), Julie Granger (Univ. Conn.)
Objective: Using field and laboratory data from a recently funded NSF EAGER award, this working group will conduct a thorough assessment of the 15N2 tracer incubation method (accuracy, error analysis, detection limit, etc.) for measuring nitrogen fixation rates and accompanying molecular methods that identify responsible diazotrophs.
Diazotroph quantitative PCR (meta)datasheet
DiaRy: Diazotroph primer-probe libraRy for qPCR
Working Group Members
Mar Benavides - Univ. of Copenhagen, Denmark
Ilana Berman-Frank - Bar-Ilan Univ.
Sophie Bonnet - Mediterranean Institute of Oceanology
Annie Bourbonnais - Univ. South Carolina
Bonnie Chang - Univ. of Washington
Dreux Chappell - Old Dominion Univ.
Anne Dekas - Stanford Univ.
Wally Fulweiler - Boston Univ.
Rosie Gradoville - Oregon State Univ.
Julie Granger - Univ. of Connecticut
Bethany Jenkins - Univ. Rhode Island
Todd Kana - Horn Point Laboratory
Angela Knapp - Florida State Univ.
Etai Landou - Bar-Ilan Univ.
Pia Moisander - Univ. of Massachusetts - Dartmouth
Wiebke Mohr - Max Planck Institute for Marine Microbiology
Margie Mulholland - Old Dominion Univ.
Eric Raes - Alfred Wegener Institute for Polar and Marine Research
Lasse Riemann - Univ. of Copenhagen
Corday Seldon - Old Dominion Univ.
Kendra Turk-Kubo - Univ. California Santa Cruz
Angelicque White - Oregon State Univ.
Sam Wilson - Univ. of Hawaii - Honolulu
Tests of different methods of introducing 15N2 investigated by Mahaffey, Rees and Kitidis (2013) - PDF
2012 Kiel Workshop Report - description of problem, summary and minutes, prepared by Jonathan Zehr, Wiebke Mohr, Rebecca Langlois - PDF
Spreadsheet - calculates nitrogen fixation rates and corresponding limits of detection (LOD) for each measured rate.